Varying label density allows artifact-free analysis of membrane-protein nanoclusters
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چکیده
منابع مشابه
Label-free proteomic analysis of the hydrophobic membrane protein complement in articular chondrocytes: a technique for identification of membrane biomarkers.
CONTEXT There is insufficient knowledge about the chondrocyte membranome and its molecular composition. OBJECTIVE To develop a Triton X-114 based separation technique using nanoLC-MS/MS combined with shotgun proteomics to identify chondrocyte membrane proteins. MATERIALS AND METHODS Articular chondrocytes from equine metacarpophalangeal joints were separated into hydrophobic and hydrophilic...
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ژورنال
عنوان ژورنال: Nature Methods
سال: 2016
ISSN: 1548-7091,1548-7105
DOI: 10.1038/nmeth.3897